Anti-BMP-9 (GDF-2)

Application

Research CategoryNeuroscience

Anti-BMP-9 (GDF-2), Cat. No. ABN1024, is a highly specific rabbit polyclonal antibody that targets Bone morphogenetic protein 9 (Growth/differentiation factor 2) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BMP-9 (GDF-2) in human liver and human liver cancer tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Growth/differentiation factor 2 (UniPort: Q9UK05; also known as GDF-2, Bone morphogenetic protein 9, BMP-9) is encoded by the GDF2 (also known as BMP9) gene (Gene ID: 2658) in human. BMP-9 is a member of the TGF-beta family that forms a signaling complex with two type 1 and two type II receptors. It is synthesized with a signal peptide of (aa 1-22) and a propeptide (aa 22-319). The signal peptide is cleaved in the mature protein. The propeptide of BMP-9 does not interfere with the biological activity of BMP-9 and is believed to remain associated with the mature peptide. BMP9 is constitutively secreted from the liver in an active form into the circulation. BMP-9 signals through high affinity activing receptor-like kinase 1 (ALK1) in endothelial cells and maintains endothelial homeostasis. It is also considered as a potent osteogenic signaling factor in mesenchymal stem cells. BMP-9 is also known to induce an increase in collagen, fibronectin, and connective tissue growth factor expression associated with Smad 1/5/8, Smad 2/3, and Erk1/2 activation. It is also reported to promote the acquisition of the cholinergic phenotype in basal forebrain cholinergic neurons during development and protects these neurons. Defects in BMP9 gene are known to cause Telangiectasia, hereditary hemorrhagic disease that leads to vascular dysplasia leading to dilation of permanent blood vessels and arteriovenous malformations of skin, mucosa, and viscera and causes recurrent epistaxis and gastro-intestinal hemorrhage (Ref.: Wei, Z et al. (2014). J. Biol. Chem. 289 (45); 31150 31159; Munoz-Felix, JM et al. (2016). Cell Signal 28(9): 1252-1261).

Immunogen

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 110 amino acids from the C-terminal region of human Bone morphogenetic protein 9 (BMP-9).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH7.4 with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected BMP-9 (GDF-2) in 10 µg of human liver tissue lysate.

Specificity

This rabbit polyclonal antibody detects human Bone morphogenetic protein 9 (BMP-9). It targets an epitope within 110 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed; 47.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).